nanoTools for Research
29.09.2008
Dear Scientist,

Please find in this Newsletter:
The Latest Product News on nanoTools' Autophagy-related Monoclonal Antibodies


(1)

0231-100/LC3-5F10

(A)                                   (B)

Endogenous LC3 punctae detected with anti-LC3 monoclonal antibody.


The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 signals of increase after induction of autophagy by vinblastine-stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10

(Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).

(2)

0231-100/LC3-5F10

0260-100/LC3-2G6
0261-100/LC3-5H3
0240-100/Beclin-12B4


                
Immunoblot analysis of LC3 and Beclin using anti-LC3- and anti-Beclin monoclonal antibodies.

Whole cell lysates of Neuro2A  cells were applied to SDS-PAGE. The immunoblots were probed with the indicated monoclonal antibodies for 1h at RT and developed by ECL (exp. time 30 sec).

M: Molecular weight standard
1: 0260-100/LC3-2G6     (0.5 µg/ml)
2: 0261-100/LC3-5H3     (1    µg/ml)
3: 0240-100/Beclin-12B4 (0.5 µg/ml)

(3)

0262-100/ATG5-7C6
0263-100/ATG5-11C3

  
(4)

0250-100/Rictor-1G11

 

(5)

0255-100/Raptor-10E10



Detection of endogenous Rictor and Raptor
Whole cell lysates of serum-starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membrane. Immunoblot were probed with (4) mab Rictor-1G11 (0.5µg/ml) and (5) mab Raptor-10E10 (0.5µg/ml) for 1h at RT.

1: HeLa, 2: HepG2; 3: HEK293; 4: SH-SY5Y; 5: MDCK; 6: PC12; 7: CMT 93; 8: Neuro 2A, 9: NIH 3T3