LC3 (microtubule-associated protein 1 light chain 3):Monoclonal antibody LC3-5H3 for the detection of endogenous LC3A,B,C in immunoblotting. Monoclonal antibody LC3-5F10 (also available in biotinylated form) for the detection of endogenous LC3B in immunocytochemistry as well as in immunoblotting.
See also the new improved anti-LC3B antibody LC3-2G6 for immunoblot applications.
For product details please click here:
Beclin:Monoclonal antibody Beclin-12B4 for the detection of endogenous Beclin-1 in immunoblotting as well as in immunocytochemistry.
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Atg5 (autophagy-related gene 5):Monoclonal antibodies Atg5-7C6 and Atg5-11C3 for the detection of endogenous Atg5 in immunoblotting, immunocytochemistry as well as in flow cytometry.
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Endogenous LC3 punctae detected with anti-LC3 monoclonal antibody.
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 signals of increase after induction
of autophagy by vinblastine-stimulation for 2 hours (B). Cells were
fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10
(Images by courtesy of I. Ciechomska and A.
Tolkovsky, University of Cambridge, UK).
Immunoblot analysis of LC3 and Beclin using anti-LC3- and anti-Beclin monoclonal antibodies.
cell lysates of Neuro2A cells were applied to SDS-PAGE. The
immunoblots were probed with the indicated monoclonal antibodies for 1h
at RT and developed by ECL (exp. time 30 sec).
M: Molecular weight standard
1: 0260-100/LC3-2G6 (0.5 µg/ml)
2: 0261-100/LC3-5H3 (1 µg/ml)
3: 0240-100/Beclin-12B4 (0.5 µg/ml)
New Autophagy- and PKB/Akt-Signalling related Monoclonal Antibodies:Monoclonal antibodies Raptor-10E10 and Rictor-1G11 for the detection of endogenous Raptor and Rictor in immunoblotting.
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Detection of endogenous Rictor and Raptor
Whole cell lysates of serum-starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membrane. Immunoblot were probed with (4) mab Rictor-1G11 (0.5µg/ml) and (5) mab Raptor-10E10 (0.5µg/ml) for 1h at RT.
1: HeLa, 2: HepG2; 3: HEK293; 4: SH-SY5Y; 5: MDCK; 6: PC12; 7: CMT 93; 8: Neuro 2A, 9: NIH 3T3