Products:

 

Akt Signalling

 

The PKB (RAC/akt) protein kinases are a family of second messenger serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-form being the cellular homologue of the v-akt oncogene.
Stimulation of cells with growth factors leads to activation of PKB/akt by a phosphoinositide-3-
kinase dependent signal transduction pathway.

Monoclonal antibodies against:

PKB/Akt, C-Terminus
PKBalpha/Akt1
PKBbeta/Akt2
PKB/Akt, dephospho-Ser 473
PKB/Akt, phospho-Ser 473

Raptor
Rictor

eNOS, phospho-Ser 1177
BAD
GSK3alpha
GSK3alpha, phospho-Ser 21
GSK3beta
GSK3beta, phospho-Ser 9


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News

pfeil New Autophagy-related Monoclonal    Antibodies
Monoclonal antibodies Atg5-7C6 and Atg5-11C3 for the detection of endogenous Atg5 in immunoblotting, immunocytochemistry as well as in flow cytometry.



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0262-100/ATG5-7C6

0263-100/ATG5-11C3


Monoclonal antibody LC3-5H3 for the detection of endogenous LC3A, B, C (microtubule-associated
protein 1 light chain 3 A,B,C) in immunoblotting.

For detailed product information please click here: 0261-100/LC3-5H3

Monoclonal antibody Beclin-12B4 for the detection of endogenous Beclin in immunoblotting.

For further information please click here: 0240-100/Beclin-12B4

New improved Anti-LC3 Monoclonal antibody for Western-Blot Applications

For further information please click here : 0260-100/LC3-2G6

Monoclonal antibody LC3-5F10
for the detection of endogenous
LC3B (microtubule-associated
protein 1 light chain 3B) in immunocytochemistry (see images below) as well as in immunoblotting.

For immunocytochemistry please see also latest citation: J.Biol. Chem., 282 (33),24131-24145, August 17, 2007.

0231-100/LC3-5F10


(A)                            (B)

Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine-stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10 (Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).

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pfeil New PKB/Akt-Signalling-related    Monoclonal Antibodies
Monoclonal antibodies Raptor-10E10 and Rictor-1G11 for the detection of endogenous Raptor and Rictor in immunoblotting.

For further information please click here:
0250-100/Rictor-1G11
0255-100/Raptor-10E10