Immunocytochemistry

(A) (B)
Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A)
COS7 cells, whereas punctuated signals of LC3 increase after induction
of autophagy by vinblastine-
stimulation for 2 hours (B). Cells were
fixed in paraformaldehyd, followed by methanol. Permeabilization was
performed with 0.3% Triton X-100 and endogenous LC3 subsequently
detected by MAb 5F10.
(Images by courtesy of I. Ciechomska and A.
Tolkovsky, University of Cambridge, UK).