SHOP: Autophagy

monoclonal antibody to LC3 (microtubule-associated protein 1 light chain 3B)

Order No. Applications Size Price
0231-100/LC3-5F10 ICC, WB 100 µg 316,00 €
 Add Product add to basket
Order No.: 0231-100/LC3-5F10
Clone Name: 5F10
Specificity: LC3-I (18 kDa); LC3-II (16 kDa)
Isotype: IgG1
Size: 100 µg
Modifications: none
Positive Control: Cell lysate from untreated Neuro 2A (#0911)
Applications: ICC, WB
Price: 316 €
Host: Mouse
Immunogen: synthetic peptide conjugated to hemocyanin
Epitope: N-terminus of LC3-B
Species Reactivity:
Human Mouse Rat Dog Hamster
yes yes yes yes yes
Synonyms:

Related Products:
  Order No. Clone Name Specificity
  0231-100BIOTIN/LC-3-5F10 5F10 LC3-I (18kDa); LC3-II (16kDa)
  0260-100/LC3-2G6 2G6 LC3-I (18kDa); LC3-II (16kDa)
  0261-100/LC3-5H3 5H3 LC3-I (18 kDa); LC3-II (16 kDa)
  0240-100/Beclin-12B4 12B4 Beclin
 
  0060-100/bA4(40)-5C3 5C3 Amyloid bA4 (1-40) (C-Terminus)
  0061-100/bA4(42)-8G7 8G7 Amyloid bA4 (1-42) (C-Terminus)
  0062-100/bA4(40/42)-9F1 9F1 Amyloid bA4 (40/42) (C-Terminus)
  0064-100/bA4N-19H5 19H5 Amyloid bA4 (N-Terminus)
  0084-100/bA4N-19H11 19H11 Amyloid bA4 (N-Terminus)
  0095-100/bA4(43)-6G12 6G12 Amyloid bA4 (1-43) (C-Terminus)
  0197-100/bA4N-11H3 11H3 Amyloid bA4 (free N-Terminus)
 
  0049-100/PKB-11A11 11A11 PKB/Akt (dephospho-Ser 473)
  0108-100/PKB-11E6 11E6 PKB/Akt (phospho-Ser 473)
  0119-100/PKB-5C10 5C10 PKB/Akt (C-Terminus)
  0144-100/PKBa-5G12 5G12 PKBalpha/Akt1 (generic)
  0179-100/PKBb-8B7 8B7 PKBbeta/Akt2 (generic)
 
  0046-100/Mxi2-2F2 2F2 Mxi2 (N-Terminus)
  0041-100/SAPK1/2-9H8 9H8 SAPK1/2 (pT-P-pY; pT-G-pY)
  0190-100/SAPK1a-12C5 12C5 SAPK1alpha/jnk2 (N-Terminus)
  0200-100/SAPK1g-5D10 5D10 SAPK1gamma/jnk1 (N-Terminus)
  0034-100/SAPK2a-13D5 13D5 SAPK2a/p38a
  0035-100/SAPK2a-20B11 20B11 SAPK2a/p38a (does not bind to Mxi2)
  0053-100/SAPK2d-5H7 5H7 SAPK2d/p38d
Mouse

Further Information

Datasheet
LC3-5F10

Positive Control
Cell lysate 0911/Neuro2A/Co

Immunocytochemistry


(A)                          (B)

Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine-
stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10.
(Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).