Positive Control #1041 (LC-I, enriched cell fraction from PC3 cells)
Positive Control #1042 (LC-II, enriched cell fraction from PC3 cells)
Positive Control #0911 (Neuro 2A, untreated)
Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine-
stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10.
(Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).