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Further Information
Datasheets
LC3-5F10 Positive Control #1041 (LC-I, enriched cell fraction from PC3 cells) Positive Control #1042 (LC-II, enriched cell fraction from PC3 cells) Positive Control #0911 (Neuro 2A, untreated)
Immunocytochemistry
 (A) (B) Endogenous LC3 punctae detected with anti LC3-MAb The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine- stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10. (Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK). |
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