SHOP: Autophagy

monoclonal antibody to LC3 (microtubule-associated protein 1 light chain 3B)
Order No. Applications Size Price
0231-100/LC3-5F10 ICC, WB 100 µg 316,00 €
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Order No.: 0231-100/LC3-5F10
Clone Name: 5F10
Specificity: LC3-I (18 kDa); LC3-II (16 kDa)
Isotype: IgG1
Size: 100 µg
Modifications: none
Positive Control: Cell lysate from untreated Neuro 2A (#0911)
Applications: ICC, WB
Price: 316 €
Host: Mouse
Immunogen: synthetic peptide conjugated to hemocyanin
Epitope: N-terminus of LC3-B
Species Reactivity:
Human Mouse Rat Dog Hamster
yes yes yes yes yes
Synonyms:

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Mouse

Further Information

Datasheet
LC3-5F10

Positive Control
Cell lysate 0911/Neuro2A/Co

Immunocytochemistry


(A)                          (B)

Endogenous LC3 punctae detected with anti LC3-MAb
The majority of LC3 was diffusely localized in unstimulated (A) COS7 cells, whereas punctuated signals of LC3 increase after induction of autophagy by vinblastine-
stimulation for 2 hours (B). Cells were fixed in paraformaldehyd, followed by methanol. Permeabilization was performed with 0.3% Triton X-100 and endogenous LC3 subsequently detected by MAb 5F10.
(Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK).